高糖对培养大鼠施万细胞生长、凋亡的影响及其机制探讨

乐嫣; 朱浩; 王文进; 丁文龙

doi:10.3969/j.issn.0529-1356.2009.02. 015

解剖学报 ›› 2009, Vol. 40 ›› Issue (2) : 246-249. DOI: 10.3969/j.issn.0529-1356.2009.02. 015

论著

高糖对培养大鼠施万细胞生长、凋亡的影响及其机制探讨

乐嫣 ;朱浩; 王文进; 丁文龙*

作者信息 +

Effects and mechanism of highglucose on growth and apoptosis of Schwann cells EM>in vitro/EM>

Author information +

文章历史 +

Abstract

Objective To study the effects and possible mechanism of highglucose on rat Schwann cells growth and apoptosis EM>in vitro/EM>. Methods Schwann cells were cultured with different concentrations of glucose for 72 hours. MTT assays were used to detect the activities of SCs. Apoptosis was observed by TUNEL assay. The expressions of Bcl-2 and Bax protein were evaluated by Western blotting assay. Results In high-glucose (60mmol/L，90mmol/L) group，the activity of SCs was decreased and the rate of apoptosis was increased. The expression level of Bcl-2 in SCs was decreased while Bax expression level was increased in high glucose culture. Conclusion High glucose inhibits the growth of SCs and increases the cell apoptosis，which is possibly caused by decreasin

导出引用

乐嫣;朱浩;王文进;丁文龙. 高糖对培养大鼠施万细胞生长、凋亡的影响及其机制探讨[J]. 解剖学报. 2009, 40(2): 246-249 https://doi.org/10.3969/j.issn.0529-1356.2009.02. 015

Effects and mechanism of highglucose on growth and apoptosis of Schwann cells EM>in vitro/EM>[J]. Acta Anatomica Sinica. 2009, 40(2): 246-249 https://doi.org/10.3969/j.issn.0529-1356.2009.02. 015

中图分类号： R322.85